Expansify™ 1-Well Plate

The Expansify™ 1-Well Plate supports hundreds of million cells per plate, delivering up to 10 to 100 times higher cell yields than conventional cultureware while providing a direct path from laboratory research to translational and production-scale workflows. A single, stackable plate can replace up to fifteen T-225 flasks, reducing incubator footprint, labor, and process variability while delivering reproducible, high-density cell growth in an automation-native format.

The platform supports a broad range of applications including

• Cell line development
• General cell culture
• Post-electroporation recovery
• Automated cell culture
• T cell expansion / CAR-T production
• Stem cell expansion
• Viral vector production
• Biologics production
• Process development
• Generation of large cell populations for data-intensive biology applications (e.g., functional genomics and AI-enabled biological model training)

Expansify™ provides a common production environment for both primary and immortalized cells, whether adherent or suspension, and is compatible with extracellular matrix coatings including vitronectin, fibronectin, and laminins.

At the core of every plate is XDemics’ proprietary High-Density Cell Respiration (HDCR™) architecture. Inspired by the capillary proximity found in living tissues, HDCR™ combines a precisely engineered culture geometry with a medical-grade oxygen-permeable growth surface that delivers oxygen directly to cells across the entire culture area. By overcoming the oxygen diffusion limitations of conventional cultureware, Expansify™ plates support natural tissue-level cell densities while eliminating the need for agitation, sparging, or other shear-inducing oxygenation methods.

The Expansify™ 1-Well Plate bridges the gap between laboratory research and larger-scale production workflows. Its high-capacity format enables efficient process development, scale-up studies, and translational research while maintaining the same cellular microenvironment found throughout the Expansify™ product family.

Each plate incorporates SureShelves™, a set of precision-engineered features standard across the Expansify™ product family. SureShelves provide stable rests for pipette tips, serological pipettes, and Pasteur pipettes, while integrated volume graduations enable rapid and accurate media addition and aspiration. Cells can be imaged directly in the plate using standard laboratory microscopes without transfer or manipulation.

The Expansify™ 1-Well Plate is designed for efficient handling and stacking while maintaining compatibility with standard laboratory workflows and equipment. The platform also preserves the same HDCR™ cellular microenvironment used throughout the Expansify™ product family, enabling straightforward scale-up from research through production.

The Expansify™ 1-Well Plate is manufactured in a standard ANSI/SLAS footprint, making it compatible with automated liquid handlers, imaging systems, and plate-based instrumentation. The platform also preserves the same HDCR™ cellular microenvironment used throughout the Expansify™ product family, enabling straightforward scale-up from research through production.

For adherent primary cell culture, we recommend coating the plates with an extracellular matrix component such a vitronectin, fibronectin, laminin or others. Please see the Expansify™ Cell Culture Examples document in the Application Notes section of our website for a protocol.

Each plate includes an HDCR™ insert, polystyrene base plate, and polystyrene lid. Available in packs of 1 and 10.

For Research Use Only.